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:: Volume 12, Issue 4 (suppl1 2011) ::
J Shahrekord Univ Med Sci 2011, 12(4): 54-61 Back to browse issues page
The inhibitory effects of myrtle (Myrtus communis) extract on Herpes simplex virus-1 replication in Baby Hamster Kidney cells
Mohamaad Taghi Moradi , Ali Karimi * , Mahmood Rafieian , Soleyman Kheiri , Mojtaba Saedi
Medical Plants Research Center, Shahrekord Univ. of Med. Sci. Shahrekord , kakarimi63@yahoo.com
Abstract:   (11988 Views)
Background and aim: In recent years, following the increasing of drug resistant strain of viruses, there has been an increasing interest in the use of natural substances with antiviral activity and low level of side effects. One of these herbal medicines, myrtle, showed some therapeutic effects including antibacterial and anti viral activities. Therefore, this study was aimed to determine minimum inhibitory concentration of hydroalchoholic extract of myrtle on Herpes simplex virus-1 (HSV-1) in vitro. Methods: In this experimental study, the hydroalchoholic extract of myrtle leaves was prepared using 70% ethanol and maceration method. Baby Hamster Kidney (BHK) cells were grown in monolayer culture with Dulbeccos Modified Eagles Medium (DMEM) supplemented with 5% fetal calf serum and plated onto 48-well culture plates. Fifty percent cytotoxic concentration (CC50%) of the extract on BHK cells was determined and subsequently, 50% inhibitory concentration (IC50%) of the herbal extract on replication of HSV-1 both in extra cellular and intercellular cases were assessed. Data were analyzed using probit analysis. Results: Based on Probit analysis, CC50% of the extract was 4.96 mg/ml. Significant relationship was found between the concentration of the extract and cell death in the cell studied using the Probit model (P<0.01). Based on Probit analysis, IC50 of the extract on the virus before cellular attachment and after entering the cells were 3.1 mg/ml and 1.11 mg/ml, respectively. Based on the Probit model, by increasing the extract concentration, percentage of inhibition of cytopathic effect (CPE) was increased in both stages (P<0.05). Conclusion: Based on the findings of this study, it seems that hydroalchoholic extract of myrtle can be used as an appropriate anti-herpetic herbal medicine. However, more comprehensive studies are needed to be carried out.
Keywords: Antiviral herbal medicine, Herpes simplex virus type-1, Mode of action, Myrtus communis extract.
Full-Text [PDF 249 kb]   (2292 Downloads)    
Type of Study: Research | Subject: microbiology
Received: 2011/06/2
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Moradi M T, Karimi A, Rafieian M, Kheiri S, Saedi M. The inhibitory effects of myrtle (Myrtus communis) extract on Herpes simplex virus-1 replication in Baby Hamster Kidney cells. J Shahrekord Univ Med Sci. 2011; 12 (4) :54-61
URL: http://journal.skums.ac.ir/article-1-441-en.html


Volume 12, Issue 4 (suppl1 2011) Back to browse issues page
مجله دانشگاه علوم پزشکی شهرکرد Journal of Shahrekord University of Medical Sciences
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