:: Volume 20, Issue 2 (6-2018) ::
J Shahrekord Univ Med Sci 2018, 20(2): 51-58 Back to browse issues page
Investigating the in vitro toxicity of silica oxide nanoparticles on differentiated neuronal cells (PC12)
Elham Yektadoost , Mojtaba Falahati , Soyar Sari , Farnoosh Attar *
Biology Dept., Faculty of Food Industry and Agriculture, Standard Research Institute (SRI), Karaj, I.R. Iran , f.attar@standard.ac.ir
Abstract:   (1388 Views)
Background and aims: Silica oxide nanoparticles (SiO2-NPs) have attracted a great interest in biotechnology and medicinal fields. It has been documented SiO2-NPs can induce cytotoxicity in normal cells. However, until now, the cytotoxicity of SiO2-NPs against nervous system cells did not examine.
Methods: In this in vitro study, the activities of caspase-8 and 6 were analyzed in the PC12 cells treated with different concentrations of silica oxide nanoparticles, and was assessed by Elisa plate reader. Data were analyzed using SPSS software, and by One-way ANOVA and Student t-test.
Results: The results showed that the caspase-8 and 6 activities increased in a concentration dependent manner. Indeed, the activity of caspase-8 and 6 was induced after exposure to 1-100 µM of SiO2-NPs. However, the SiO2-NPs showed only slight toxicity in the concentration of 0.1 µM (P<0.05).
Conclusion: A mitochondrial-dependent pathway activated by caspase-8 and 6 mediated by SiO2-NPs may be involved in apoptosis induced by NPs, and therefore, cell cytotoxicity plays a role in PC12 cells.
Keywords: Silica nanoparticles, Cytotoxicity, Apoptosis, Caspase
Full-Text [PDF 605 kb]   (670 Downloads)    
Type of Study: Research | Subject: Toxicology
Received: 2017/04/5 | Accepted: 2017/06/17 | Published: 2018/06/1

XML   Persian Abstract   Print

Volume 20, Issue 2 (6-2018) Back to browse issues page