:: Volume 11, Issue 3 (autuman 2009) ::
J Shahrekord Univ Med Sci 2009, 11(3): 61-69 Back to browse issues page
Detection of mutations in exons 5-8 of the P53 gene in gastric cancer samples using PCR-SSCP in Chaharmahal va Bakhtiari province 2006-2007
Javad SafariChaleshtori , Mohammad Taghi Moradi , Efat Farokhi , MohammadAmin Tabatabaee Far , M aryM Taherzadeh , Fateme Shayesteh , Gholamereza Mobini , Mehdi Banitalbi , Gashtasb Mardani , Morteza Hashemzadeh *
Professor, Cellular and Molecular Research Center, Shahrekord Univ. of Med. Sci. Shahrekord, Iran. , mchalesh@yahoo.com
Abstract:   (11043 Views)
Background and aim: Gastric cancer is the second cause of cancer death world wide. Genetic factors including oncogens and tumor suppressor genes are always contributed in progression of this cancer. The P53 tumor suppressor gene has a broad role in the cell such as programmed cell death and stop cell replicating damaged DNA. Mutations in the P53 gene which are frequently seen in human gastric cancer impair its tumor suppression function. The aim of this study was to determine the P53 gene mutations in gastric cancer specimens in Chaharmahal va Bakhtiari Province. Methods: In this deh1ive-lab based study we investigated the P53 gene mutations in exons 5-8 in 38 paraffin embedded gastric cancer specimens. DNA was extracted following the standard phenol chloroform protocol. The P53 gene mutations were determined using PCR-SSCP procedure. Results: Band shifts were detected in all positive controls examined. However no shifted band was detected in samples from gastric cancer patients tested. Conclusion: The results of this study demonstrated that association between P53 gene mutations and gastric cancer is very low in Chaharmahal va Bakhtiari province. However we have examined a limited number of 38 gastric samples and more samples are needed to be investigated to unravel the contribution of P53 gene mutations leading to gastric cancer in this province.
Keywords: Gastric cancer Mutation P53 gene PCR-SSCP.
Full-Text [PDF 263 kb]   (2690 Downloads)    
Type of Study: Research |
Received: 2011/06/2


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Volume 11, Issue 3 (autuman 2009) Back to browse issues page