[Home ] [Archive]   [ فارسی ]  
:: Main About Current Issue Archive Search Submit Contact ::
:: Volume 19, Issue 2 (Jun, Jul 2017) ::
J Shahrekord Univ Med Sci 2017, 19(2): 124-132 Back to browse issues page
Genetic linkage analysis of DFNB24 locus in a group of families with autosomal recessive non-syndromic hearing loss in Khouzestan province of Iran
Ladan Sadeghian , Mohammad Amin Tabatabaiefar , Narges Zarepour , Morteza Hashemzadeh-chaleshtori *
Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, I.R. Iran , mchalesh@yahoo.com
Abstract:   (774 Views)

Background and aims: Hearing loss (HL) is the most common sensorineural disorder affecting 1 in 1000 newborns. About 70% of genetic HL is classified as nonsyndromic deafness. Over 100 non-syndromic loci have been identified in autosomal recessive nonsyndromic hearing loss (ARNSHL). The aim of this study is identifying genetic cause of deafness by investigating genetic linkage analysis for DFNB24 locus (RDX gene) in ARNSHL families.

Methods: In this descriptive-experimental study, 400 samples from 25 Iranian consanguinity families with 3 or more patients, segregating as an ARNSHL, were selected from Khuzestan. Totally, 23 families were negative for GJB2 mutations (DFNB1) and were included. Six STR (Short Tandem Repeat) markers were selected, PCR amplification was done, and genotyping was performed by electrophoresis PCR product on polyacrylamide gel (PAGE). Several software tools such as Easy Linkage, SimWalk and HaploPainter were used for linkage analysis.

Results: Following the study of genetic linkage of DFNB24 in hearing loss population of Kuzestan province, our findings showed no families to be linked to the DFNB24 locus and deafness.

Conclusion: Results of the present study showed that RDX mutations may have little role in the etiology of deafness in this province.

Keywords: Genetic linkage, Autosomal recessive non-syndromic hearing loss, DFNB24 locus.
Full-Text [PDF 652 kb]   (198 Downloads)    
Type of Study: Research | Subject: genetic
Received: 2016/06/26 | Accepted: 2017/01/30 | Published: 2017/06/11
Send email to the article author

Add your comments about this article
Your username or Email:

Write the security code in the box >



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sadeghian L, Tabatabaiefar M A, Zarepour N, Hashemzadeh-chaleshtori M. Genetic linkage analysis of DFNB24 locus in a group of families with autosomal recessive non-syndromic hearing loss in Khouzestan province of Iran . J Shahrekord Univ Med Sci. 2017; 19 (2) :124-132
URL: http://journal.skums.ac.ir/article-1-2977-en.html
Volume 19, Issue 2 (Jun, Jul 2017) Back to browse issues page
مجله دانشگاه علوم پزشکی شهرکرد Journal of Shahrekord University of Medical Sciences
Persian site map - English site map - Created in 0.052 seconds with 795 queries by yektaweb 3535