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:: Volume 11, Issue 3 (autuman 2009) ::
J Shahrekord Univ Med Sci 2009, 11(3): 70-76 Back to browse issues page
The synergic effects of rapamycin and extremely low frequency electromagnetic field on angiogenesis
Saeedeh Zafar-Balanezhad * , Kazem Parivar , Javad Baharara , Homa Mohseni-Koochesfahani , Alireza Ashraf
PhD Student, Biology Dept., Islamic Azad University Branch, Sciences & Research Branch, Tehran , mojgan_zafar@yahoo.com
Abstract:   (15240 Views)
Background and aim: Angiogenesis the formation of new blood vessels is a dynamic and complex activity which is needed for embryogenesis and other physiological processes. However in many pathological conditions such as solid tumor progression the disease appears to be associated with persistent up-regulated angiogenesis. In this research we used 0.04 T (tesla) electromagnetic field as a synergic treatment with rapamycin on angiogenesis. Methods: In this experimental study 70 Ross fertilized eggs were randomly divided into 5 groups as following: 1) control 2) sham-exposed 3) a group treated with rapamycin 4) a group treated with electromagnetic field and 5) a group treated with both rapamycin and electromagnetic field. In day 2 a window was opened on eggs in sterile condition. In day 8 a gelatin sponge was placed on chorioalantoic membrane (CAM) and was soaked with 5 μl rapamycin in group 3 and group5. Groups 4 and group 5 were placed in 400 Gauss magnetic field for 4 hours in day 10. In day 12 CAMs were examined and photographed by research photo-stereomicroscope in all cases. Data were analyzed using ANOVA and Mann-Whitney tests. Results: Comparison between average number and length of vessels in controls and sham-exposed didn’t show any significant differences. In group 3 and 4 a significant decrease was shown in the average number and length compared with controls. Finally comparison between group 3 and group 5 showed a significant decrease in the average number and length of vessels in group 5 which had been treated with both rapamycin and 400 (Gauss) G electromagnetic field. Conclusion: The results of this study showed that 0.04 T magnetic field has an inhibitory effect on angiogenesis in CAM and can enhance the effect of rapamycin as an anti-angiogenesis drug.
Keywords: Angiogenesis Chorioalantoic membrane Electro magnetic field Rapamycin.
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Type of Study: Research |
Received: 2011/06/2
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Zafar-Balanezhad S, Parivar K, Baharara J, Mohseni-Koochesfahani H, Ashraf A. The synergic effects of rapamycin and extremely low frequency electromagnetic field on angiogenesis. J Shahrekord Univ Med Sci. 2009; 11 (3) :70-76
URL: http://journal.skums.ac.ir/article-1-295-en.html


Volume 11, Issue 3 (autuman 2009) Back to browse issues page
مجله دانشگاه علوم پزشکی شهرکرد Journal of Shahrekord University of Medical Sciences
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