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:: Volume 19, Number 2 (6-2017) ::
J Shahrekord Univ Med Sci 2017, 19(2): 98-108 Back to browse issues page
Molecular analysis of genes of ESBL )SHV.TEM.CTX) in Shigella Sonnei isolated from clinical samples by PCR
Shadi Mosadegh, Gholamali ‎ Moradli ‎ *
‎ Microbiology Dept., Saveh Branch, Islamic Azad University, Saveh, I.R. Iran. , moradli.mic@gmail.com
Abstract:   (601 Views)

Background and aims: Shigella is one of the most common causes of dysentery and sometimes mortality specially in children and immunocopromised patients and the occurrence of drug resistance, makes it difficult the choice of appropriate antibiotics to treat shigellosis. The aim of this study was to define the prevalence of blaCTX-M, blaTEM and blaSHV  in Shigella Sonnei isolated from patients with diarrhea by Multiplex-PCR.

Methods: In this cross-sectional study, a total of 60 Shigella Sonnei isolates of private laboratories and hospitals in Northern Tehran different ages from diarrhea samples were collected and cultured in specific environments and with biochemical and serological confirmation. To identify resistan strains t to beta test was conducted phenotypic combine disk. The presence of blaCTX-M, blaTEM and blaSHV  genes was evaluated by specific primers and Multiplex-PCR.

Results: The highest frequency resistance to the antibiotics penicillin and erythromycin was 96.6% and 95%, respectively, and prevalence maximum sensitivity has been reported to ciprofloxacin and imipenem with 70%. In the Combine disk method, 29 strains (48.3%) were found positive to
beta-lactamase. Among 60 isolates, 39 samples (65%) had the blaTEM gene, 28 samples (46.6%) had the blaCTX-M gene and 1 sample (1.66%) had the blaSHV gene.

Conclusion: Given the high prevalence of beta-lactam antibiotics resistance genes in strains of Shigella Sonnei ‎, careful medical care and proper use of antibiotics appropriate is essential and timely to prevent the spread of resistant strains is necessary.

Keywords: ESBL, Shigella Sonnei, Multiplex-PCR.
Full-Text [PDF 954 kb]   (142 Downloads)    
Type of Study: Research | Subject: microbiology
Received: 2016/05/29 | Accepted: 2016/10/10 | Published: 2017/06/6
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Mosadegh S, Moradli ‎ G ‎. Molecular analysis of genes of ESBL )SHV.TEM.CTX) in Shigella Sonnei isolated from clinical samples by PCR. J Shahrekord Univ Med Sci. 2017; 19 (2) :98-108
URL: http://journal.skums.ac.ir/article-1-2935-en.html
Volume 19, Number 2 (6-2017) Back to browse issues page
مجله دانشگاه علوم پزشکی شهرکرد Journal of Shahrekord University of Medical Sciences
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