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:: Volume 19, Number 2 (6-2017) ::
Back to browse issues page J Shahrekord Univ Med Sci 2017, 19(2): 51-59
Evaluation of the expression of Hottip long noncoding RNA in the B16F10 murine melanoma cell line
Lale Shiri Sichani, Mojtaba Emadi- Baygi, Majdolden Rezaei, Hossein Khanahmad, Parvaneh Nikpour *
Genetics and Molecular Biology Dept., Pediatric Inherited Diseases Research Center, Research Institute for Primordial Prevention of Non-communicable Disease, Isfahan University of Medical Sciences, Isfahan, I.R. Iran , pnikpour@med.mui.ac.ir
Abstract:   (219 Views)

Background and aims: Melanoma is the most dangerous form of skin cancer. One of the remarkable cancer- causing factors is non-coding RNAs including lncRNAs (long noncoding RNA). lncRNAs have more than 200 bases and act as a key regulator of cancer progression. HOTTIP (HOXA transcript at the distal tip) is an intergenic lncRNA that is transcribed from the 5' end of the HOXA locus and activates the genes of the 5' end of this locus. The aim of this study was to evaluate the expression of this lncRNA in the B16F10 murine melanoma cell line.

Methods: In this study, the expression of Hottip gene was studied on B16F10 murine melanoma cell line using qualitative RT-PCR. Total RNA was extracted and cDNA was synthesized. Hottip and β2m genes were amplified using designed specific primers.

Results: The result showed that Hottip did not express in the B16F10 murine melanoma cell line.

Conclusion: In contrast to the previous studies revealing increased expression of HOTTIP in human cancers, murine Hottip does not express in the B16F10 murine melanoma cell line.

Keywords: Melanoma, lncRNA, HOTTIP, B16F10 cell line.
Full-Text [PDF 1042 kb]   (83 Downloads)    
Type of Study: Research | Subject: genetic
Received: 2016/02/17 | Accepted: 2016/07/25 | Published: 2017/06/4
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Shiri Sichani L, Emadi- Baygi M, Rezaei M, Khanahmad H, Nikpour P. Evaluation of the expression of Hottip long noncoding RNA in the B16F10 murine melanoma cell line. J Shahrekord Univ Med Sci. 2017; 19 (2) :51-59
URL: http://journal.skums.ac.ir/article-1-2808-en.html
Back to browse issues page Volume 19, Number 2 (6-2017)
مجله دانشگاه علوم پزشکی شهرکرد Journal of Shahrekord University of Medical Sciences
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