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:: Volume 10, Issue 4 (suppl1 2009) ::
J Shahrekord Univ Med Sci 2009, 10(4): 32-37 Back to browse issues page
Comparison of the antimicrobial effects of the ethanolic and aqueous extracts of Scrophularia striata on Escherichia coli O157:H7 in vitro
Farhad Sharafati-Chaleshtori * , Reza Shrafati-Chaleshtori , Mariam Momeni
, E-mail:sharafati33@yahoo.com
Abstract:   (11121 Views)
Background: Escherichia coli O157:H7 is one of the most important agents causing intestinal diseases. It is transmitted usually by contaminated food and particularly meat beef. Since chemically synthesized drugs have been shown to have undesirable side effects, in recent years, it is recommended to use natural materials for controlling and treatment of different kinds of infection. Therefore, the aim of this study was to compare the antimicrobial effects of the ethanolic and aqueous extracts of Scrophularia striata on Escherichia coli O157:H7. Methods: In this experimental study, ethanolic and aqueous extracts of Scrophularia striata were collected and their antibacterial activities on Ecoli O157:H7 were tested using the agar-diffusion method. Subsequently, using macro-dilution method, both minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract were tested against the bacterium. Amikacin (30µg) was used as an antimicrobial reference. Results: It was shown that the growth of Ecoli O157:H7 in-vitro was inhibited by the ethanolic extract of Scrophularia striata in both the agar-diffusion and macro-dilution methods. However, its aqueous extract was not effective against the bacterium. The amount of MIC and MBC of the extract were 90 and 100 mg/ml, respectively. Conclusion: The results showed that Scrophularia striata has antibacterial effects on Ecoli O157:H7. Thus, more investigation need to be done for the purification, identification, and quantification of active components presented in the ethanolic extract of Scrophularia striata.
Keywords: Antimicrobial effects, Scrophularia striata, Ecoli O157:H7.
Full-Text [PDF 169 kb]   (2340 Downloads)    
Type of Study: Research |
Received: 2011/06/2
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Sharafati-Chaleshtori F, Shrafati-Chaleshtori R, Momeni M. Comparison of the antimicrobial effects of the ethanolic and aqueous extracts of Scrophularia striata on Escherichia coli O157:H7 in vitro. J Shahrekord Univ Med Sci. 2009; 10 (4) :32-37
URL: http://journal.skums.ac.ir/article-1-247-en.html


Volume 10, Issue 4 (suppl1 2009) Back to browse issues page
مجله دانشگاه علوم پزشکی شهرکرد Journal of Shahrekord University of Medical Sciences
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