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:: Volume 18, Issue 1 (Apr,May 2016) ::
J Shahrekord Univ Med Sci 2016, 18(1): 73-80 Back to browse issues page
Identification of Salmonella serotypes in foodborne outbreaks by sequencing of ITS region of 16S-23SrRNA
Mohammad Mehdi Soltan Dallal * , Mahya Khalilian , Hossein Masoumi Asl , Ronak Bakhtiari , Abolfazl Davoodabadi , Zahra Rajabi
Division of Food Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences. Tehran, Iran , msoltandallal@gmail.com
Abstract:   (3053 Views)

Background and aim: Foodborne diseases are a major problem of modern society. The epidemiological investigations in many parts of the world had been shown that infections caused by Salmonella serovars are increasing. Therefore, the identification of Salmonella species to serovars level is in specific importance. In addition, the emergence of drug resistance among these serovars also has become a major global concern. So, the knowledge of the resistance patterns of Salmonella could be very useful for the treatment of such foodborne diseases. The aim of this study was to determine Salmonella serotypes in foodborne outbreaks by sequencing of ITS region of 16S-23SrRNA gene.

Methods: This was a cross- sectional study, in total, 173 outbreaks in different provinces of Iran during one year were selected. In total, 614 diarrheal specimens from patients in outbreaks were tested for the isolation of Salmonella. Identification of Salmonella was carried out by 16SrRNA method and antibiotic susceptibility was performed by standard methods.

Results: Of 18 Salmonella isolated from happened outbreaks, 16 cases (88.9%) were Salmonella enteridis and 2 cases (11.1%) Salmonella paratyphi A, respectively. All isolates were sensitive to ceftazidime, and high resistance to tested antibiotics was seen with nalidixic acid (77.8%) in 14 samples.

Conclusion: Prevalence of antibiotics resistance bacteria which is major threat for human health is increasing rapidly. Antibiotic resistance of Salmonella strains also are increasing. Therefore, knowledge of antibiotic resistance pattern to Salmonella serovars will help in treatment of foodborne due to Salmonella infections.

Keywords: Salmonella, Foodborne diseases, Outbreak, Diarrhea, Antibiotic susceptibility.
Full-Text [PDF 787 kb]   (730 Downloads)    
Type of Study: Research | Subject: microbiology
Received: 2015/04/6 | Accepted: 2015/10/6 | Published: 2016/04/6
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Soltan Dallal M M, Khalilian M, Masoumi Asl H, Bakhtiari R, Davoodabadi A, Rajabi Z. Identification of Salmonella serotypes in foodborne outbreaks by sequencing of ITS region of 16S-23SrRNA. J Shahrekord Univ Med Sci. 2016; 18 (1) :73-80
URL: http://journal.skums.ac.ir/article-1-2397-en.html


Volume 18, Issue 1 (Apr,May 2016) Back to browse issues page
مجله دانشگاه علوم پزشکی شهرکرد Journal of Shahrekord University of Medical Sciences
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