در حال انتقال سرور
   [Home ] [Archive]   [ فارسی ]  
:: Main About Current Issue Archive Search Submit Contact ::
:: Volume 17, Issue 3 (Aug, Sep 2015) ::
J Shahrekord Univ Med Sci 2015, 17(3): 48-56 Back to browse issues page
Damage intensity of gallic acid on prostatic cancer cells lineDu145 by alkaline electrophoresis
Javad Saffari-Chaleshtori , Mostafa Gholami , Mahnaz Keloushadi , Ehsan Heidari , Esfandiar Heidarian *
Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, I.R. Iran , j_saffari@yahoo.com
Abstract:   (6235 Views)
Background and aims: Prostatic cancer is one of the most widespread cancers in men in all over the world. There are many reagents that cause to prostate cancer, but many factors recognized to prevent or treat this cancer. This capacity is in anti-oxidants and, especially in polyphenol compounds like Gallic acid. This study was aimed to investigate the effect of gallic acid on Du145 cells line in prostatic cancer by alkaline electrophoresis technique. Methods: In this Laboratory experimental study, MTT assay was used to determine the viability of Du145 cells line in the adjacent of different concentrations of gallic acid and IC50. Three concentrations around the IC50 of Gallic acid were treated on cells for 48 hours. Comet pictures have been analyzed using CASP software after alkaline electrophoresis. Results: 35 µM of Gallic acid for Du145 cells was determined based on probit model with IC50 with MTT test. Rate of tail to head in alkaline electrophoresis for three concentrations around the IC50 (25, 30 and 35) µM of Gallic acid was 6.7±1.3, 13.8±2.6 and 69.4±1.2 percent, respectively. Conclusion: Gallic acid is one of the most powerful anti-oxidants that can reduce the growth of Du145 cells in prostatic cancer and inhibits the cell growth with destroy their genome and induce the apoptosis.
Keywords: Prostatic cancer, Du145 cells line, Gallic acid, Comet assay
Full-Text [PDF 251 kb]   (1143 Downloads)    
Type of Study: Research | Subject: genetic
Received: 2014/11/16 | Accepted: 2015/01/20 | Published: 2015/07/24
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Saffari-Chaleshtori J, Gholami M, Keloushadi M, Heidari E, Heidarian E. Damage intensity of gallic acid on prostatic cancer cells lineDu145 by alkaline electrophoresis. J Shahrekord Univ Med Sci. 2015; 17 (3) :48-56
URL: http://journal.skums.ac.ir/article-1-2226-en.html


Volume 17, Issue 3 (Aug, Sep 2015) Back to browse issues page
مجله دانشگاه علوم پزشکی شهرکرد Journal of Shahrekord University of Medical Sciences
Persian site map - English site map - Created in 0.06 seconds with 31 queries by YEKTAWEB 3792