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:: Volume 17, Issue 3 (Aug, Sep 2015) ::
J Shahrekord Univ Med Sci 2015, 17(3): 93-101 Back to browse issues page
Antileishmania activity of Levandula officinalis essence against Leishmania major in in vitro media
Mojtaba Bonyadian * , Hossein Hejazi , Hamidreza Azizi , Saeed Habibian , Emad Sayahi
Health Dept., Shahrekord University, Shahrekord, I.R. Iran , boniadian@vet.sku.ac.ir
Abstract:   (3303 Views)
Background and aims: Recently with the increase of drug resistance in curing diseases, especially parasitic diseases, and also due to the side effects of chemical compositions, the use of herbal drugs in curing parasitic diseases has increased significantly throughout the world. The present study was designed and performed to investigate the antileishmania effects of Levandula essence and to compare it with glucantime. Methods: In this experimental study, first the essential oil was extracted from the fresh dried and powdered plant by steam distillation method. Then, essentials with the concentration of 0.5, 1.5, 5, 10, 15 and 20 percent and glucantime with the concentration of 33.8% were added to Leishmania major culture media, separately. The media contained 1 × 106 promastigotes of parasite per milliliter. The number of alive parasites, after adding the essential and the drug at the times of 24, 48, 72 hours, were counted by the 10% trypan blue vital staining and the use of Neubauer lam. Results: The results showed that the proliferation rate of promastigotes after adding the plant essential declined significantly compared to the control group (P<0.05). In concentration of 10% or more, the leishmaniocidal effect was also observed in a way that in hour 72 no viable parasite was observed in the mentioned groups. Also, in concentration of 0.5, 1.5 and 5 percent, a significant difference was observed in the number of viable parasites compared to the chemical drug group (P<0.05). Conclusion: This study indicates that Levandula essential has inhibitory and lethal effects on the promastigotes form of Leishmania major in in vitro. Therefore, this is suggested that more studies is conducted in order to identify the antileishmaniosis of Levandula on the amastigote form of parasite.
Keywords: Essence, Levandula plant, Leishmania major
Full-Text [PDF 266 kb]   (1502 Downloads)    
Type of Study: Research | Subject: medical plants
Received: 2014/04/7 | Accepted: 2014/10/6 | Published: 2015/07/26
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Bonyadian M, Hejazi H, Azizi H, Habibian S, Sayahi E. Antileishmania activity of Levandula officinalis essence against Leishmania major in in vitro media. J Shahrekord Univ Med Sci. 2015; 17 (3) :93-101
URL: http://journal.skums.ac.ir/article-1-1967-en.html


Volume 17, Issue 3 (Aug, Sep 2015) Back to browse issues page
مجله دانشگاه علوم پزشکی شهرکرد Journal of Shahrekord University of Medical Sciences
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