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:: Volume 17, Issue 2 (Jun, Jul 2015) ::
J Shahrekord Univ Med Sci 2015, 17(2): 105-118 Back to browse issues page
The effect of carvacrol on the interleukin-6 gene expression and cell signaling proteins in prostatic cancer cell line DU-145
Mahnaz Keloushadi , Esfandiar Heidarian * , Keihan Ghatreh-Samani , Mahmoud Rafieian-Kopaei
Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, I.R. Iran , heidarian46@yahoo.com
Abstract:   (7378 Views)
Background and aims: Interleukin-6 (IL-6) causes anti-apoptotic and drug-resistant effects in prostate cancer cells. Carvacrol, an herbal antioxidant, exists in many different plants. The aim of this study was to evaluate the effects of Carvacrol on IL-6 gene expression, cellular signaling proteins, and cell emigration in DU-145 prostate cancer cells. Methods: In this experimental study, DU-145 cells were treated with various concentrations of carvacrol for 48 hours. Then, cell viability was assessed by MTT assay and the 50% inhibitory concentration (IC50) was determined. IL-6 levels at various concentrations of carvacrol were determined by ELISA kit. Il-6 gene expression and cell signaling proteins (pStat3، pErk and pAkt) were determined using real-time RT-PCR and western blot analysis, respectively. DU-145 cells migration and invasion were assessed by invasion assay test. Results: Carvacrol IC50 for DU-145 prostate cancer cells was 406.2 μM. Carvacrol at 150 μM exhibits IL-6 expression at both mRNA and protein levels, but carvacrol reduces IL-6 expression at more than 150 μM at both mRNA and protein levels in a dose-dependent manner (P<0.05). Carvacrol suppresses pStat3, pErk, and pAkt cell signaling proteins at a dose-dependent manner more than 150 μM. Also, carvacrol significantly declines cell viability and emigrational invasion ability in DU-145 prostate cancer cells (P<0.05). Conclusions: Carvacrol reduces cell growth, proliferation, and cell emigration through diminished cell signaling proteins and IL-6 gene expression, and it causes induction of apoptosis in DU-145 prostate cancer cells. Therefore, carvacrol can be considered as a useful therapeutic agent to treat prostate cancer.
Keywords: Interleukin-6, Prostate cancer, Carvacrol, Signaling proteins
Full-Text [PDF 362 kb]   (3710 Downloads)    
Type of Study: Research | Subject: biochimistery
Received: 2014/02/10 | Accepted: 2014/08/20 | Published: 2015/05/19
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Keloushadi M, Heidarian E, Ghatreh-Samani K, Rafieian-Kopaei M. The effect of carvacrol on the interleukin-6 gene expression and cell signaling proteins in prostatic cancer cell line DU-145. J Shahrekord Univ Med Sci. 2015; 17 (2) :105-118
URL: http://journal.skums.ac.ir/article-1-1893-en.html


Volume 17, Issue 2 (Jun, Jul 2015) Back to browse issues page
مجله دانشگاه علوم پزشکی شهرکرد Journal of Shahrekord University of Medical Sciences
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