:: Volume 15, Issue 6 (Supplement 2014) ::
J Shahrekord Univ Med Sci 2014, 15(6): 12-24 Back to browse issues page
The assessment of lentiviral vectors application for gene transformation in human dermal fibroblasts (HDFs)
Mehdi Allahbakhshian-Farsani , Payam Ghasemi-Dehkordi , Narges Abdian , Somayeh Khosravi-Farsani , Amin Mirzaeian , Masoud Nasri , Alireza Karimi-Taghanaki , Gashtasb Mardani , Seyedeh Amiri , Morteza Hashemzadeh Dr.
Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran , biotechnologyskums@gmail.com
Abstract:   (6203 Views)
Background and aims: Induced pluripotent stem cells (iPSC) are primary undifferentiated cells that are able to create almost any type of cells in the body. The purpose of the present study was production and transmission of TetO-FUW-OSKM lentiviral vector to human dermal fibroblast cells (HDFs) and investigation the application of this vector. Methods: In this experimental study after isolation and culture of HDF cells, TetO-FUW-OSKM lentiviral vector containing the reprogramming genes (as transfer vector) and PsPAX2 and PMDG2 vectors (as packaging plasmids) were transfected to HEK-293T cell line for virus production. The supernatants of packaging cells were harvested in 48h and 72h after transfection. Then, these viruses were transducted to HDF for reprogramming these cells. Results: The results of this study demonstrated the successful production of TetO-FUW-OSKM lentiviral vector and suitable expression of the transcription factors in HDF cells after transduction. Conclusion: According to these findings lentiviral vectors could be used for gene transmission and reprogramming of adult cells such HDF to generate iPS cells in future studies.
Keywords: HDF, TetO-FUW-OSKM lentiviral vector, Reprogramming, IPSc, RT-PCR
Full-Text [PDF 460 kb]   (2219 Downloads)    
Type of Study: Research | Subject: genetic
Received: 2013/09/22 | Accepted: 2014/03/4 | Published: 2014/03/11

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Volume 15, Issue 6 (Supplement 2014) Back to browse issues page