:: Volume 16, Issue 2 (May, Jun 2014) ::
J Shahrekord Univ Med Sci 2014, 16(2): 97-103 Back to browse issues page
Decreases of osteonectin gene expression by gallic acid in vascular smooth muscle cells
Neda Mohandes-Samani , Keihan Ghatre-Samani * , Hossein Fathpour , Effat Farokhi , Mahsa Salehian-Dehkordi
Shahrekord university , ghatreh_k@skums.ac.ir
Abstract:   (5842 Views)
Background and aims: Vascular calcification is important factor in atherosclerosis pathogenesis and osteonectin with others bone matrix proteins have regulatory function in atheroma formation and atherosclerosis progression. Studies showed that antioxidant compounds cause to decrease vascular calcification and prevent cardiovascular disease. The aim of this study was to investigate effects of gallic acid on osteonectin gene expression. Methods: In this experimental study, it was determined gallic acid half maximal inhibitory concentration (IC50) on vascular smooth muscle cells (VSMC). These cells were treated by 160,180 and 200 µM concentration of gallic acid for 48h and the control group also was considered. The total RNA was extracted and its cDNA was synthesized and then the quantity of osteonectin gene expression was measured by real time PCR. Results: Overall،160,180 and 200µM concentration of gallic acid decreased osteonectin gene expression in vascular smooth muscle cell by 1/37, 2/93 and 7/3 folds compared with the control group, respectively. Conclusion: Gallic acid reduces osteonectin gene expression therefore could reduce vascular calcification. Gallic acid could have prevented atheroma formation therefore could decrease the risk of cardiovascular diseases.
Keywords: Vascular smooth muscle cells, Gallic acid, Osteonectin, Atherosclerosis
Full-Text [PDF 261 kb]   (1374 Downloads)    
Type of Study: Research | Subject: biochimistery
Received: 2013/07/7 | Accepted: 2013/09/11 | Published: 2014/06/3


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Volume 16, Issue 2 (May, Jun 2014) Back to browse issues page