Background and aims: Pseudomonas aeruginosa is an opportunistic pathogen causing nosocomial infections and mortality, especially in patients with a weakened immune system and leads to problems inherent resistance to antimicrobial agents in the treatment of infections. The aim of this study was to evaluate the prevalence of Pseudomonas aeruginosa virulence genes in Multiplex-PCR method.

Methods: A total of 60 samples of Pseudomonas aeruginosa including 40 samples from Passargad microbiology lab collection and 20 samples of hospitals in Kerman collected and cultured in specific media and were confirmed with biochemical tests. PCR reaction was done on all samples to identify virulence genes ETA, oprL, gyrB and the species 16SrDNA.

Results: Of the 60 isolates of Pseudomonas aeruginosa, 38 samples (63.3%) contains gene gyrB and oprL, 37 samples (61.6%) had the gene ETA, and all had 60 isolate (100%) gene 16SrDNA.

Conclusion: Considering the importance of rapid detection of this bacterium due to the incidence of resistance and presence of problems in biochemical methods, concurrent identification of multiple genes by Multiplex-PCR is a sensitive and accurative way in detecting the bacteria.