Background and aims: The aim of this study, regarding the results of the previous researches and the effects of voluntary exercise on neurotrophic factors in treating PD, was to evaluate changes MANF level of rats` stratum exposed to neurotoxin injected by the stereotaxic surgery following the four weeks of treadmill running.

Methods: Twenty four rats were divided into four groups: Sham, Parkinson control, Parkinson exercise, and healthy control. Exercise group exercised for 4 weeks, 5 days per week and 2
15-minute sessions having at least 1 h interval. The purpose of injecting 6-OHDA into the brain striatum was to create an experimental model of PD. Three weeks after the injection of
6-hydroxydopamine (6-OHDA), Apo morphine rotational test was carried out in order to verify the rats with Parkinson latest. MANF levels in the striatum were measured by ELISA. Data was analyzed by one-way analysis of variance (ANOVA) and Tukey post-hoc test.

Results: The findings showed that there is a significant difference in the striatum MANF level of Parkinson control group (26.91±9 pg/mg) compared to the healthy control group (45.22±2 pg/mg) (P≤0.05). Furthermore, the striatum MANF level in Parkinson exercise group (29.35±2 pg/mg) had an increase in comparison with the Parkinson control group (26.91±9 pg/mg), but the difference was not significant (P=0.997).

Conclusion: This research has shown that performing treadmill running program cannot increase the MANF level of striatum. Therefore, we cannot decisively consider a neural protective role for this training protocol and it necessitates further studies.