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Protective effect of vitamin C against hemolytic anemia-induced changes in small intestine histoarchitecture of phenylhydrazine-treated mice
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| Hojjat Anbara , Rasuol Shahrooz , Ali Shalizar Jalali , Mazdak Razi , Ali Kalantari Hesari |
| Assistant Prof. Urmia University , ali_shalizar@yahoo.com |
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Abstract: (3323 Views) |
| Background and aims: Hemolytic anemia-induced hypoxia can lead to multi-organ dysfunctions. The aim of the present study was to explore the efficacy of vitamin C as an antioxidant agent against hemolytic anemia-induced changes in small intestine histoarchitecture of phenylhydrazine (PHZ)-treated mice. Methods: In this experimental study, adult male mice were randomly assigned to four groups of eight mice each. PHZ was administered to two groups of mice at a dose of 60 mg/kg per 48 hours intraperitoneally for 35 days. One of these groups received vitamin C (250 mg/kg per day) intraperitoneally four hours before PHZ administration. A vehicle-treated control group and a vitamin C control group were also included. 24 hours after the last treatment, desired segments of small intestines were dissected out and subjected to histological processing and morphometric parameters were evaluated. Results: PHZ caused significant decreases in villi width of duodenum and jejunum, crypts depth of duodenum, distribution rate of the goblet cells in ileal villi and height of villi in all segments of small intestine. Vitamin C markedly improved all changes in the aforementioned parameters. Conclusion: Vitamin C could ameliorate hemolytic anemia-induced histological injuries in mouse small intestine. |
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| Keywords: Hemolytic anemia, Phenylhydrazine, Vitamin C, Small intestine, Mouse |
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Full-Text [PDF 812 kb]
(909 Downloads)
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Type of Study: Research |
Subject:
histology
Received: 2014/12/14 | Accepted: 2015/01/21 | Published: 2015/09/23
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