Background and aims: Ovariectomy is a standard experimental model of menopause in rodent to investigate postmenopausal women . The aim of this study was to evaluate effects acute and chronic estrogen deficiency on lipid profile and glucose serum in ovariectomized (OVX) rats.

Methods: In this experimental study, Twenty-four adult female Wistar rats were divided into three groups of eight rats. The first group: sham-control, Second group: ovariectomized rats (for five weeks), Third group: ovariectomized rats (for fifty five weeks). Blood samples were taken  from the right cardiac ventricle in each group at the end of the study.  Fasting blood glucose and Lipid profile biomarkers such as total cholesterol, triglyceride, HDL cholesterol and LDL cholesterol were determined using enzymatic procedure. VLDL and Atheroginc index were calculated according to computational procedures.

Results: The results indicated that short time ovariectomy cause increase glucose,  triglyceride, high density lipoprotein, low density lipoprotein ,TG/ HDL ratio and Atherogenic index than the long term ovariectomized rats .

Conclusion: These results suggested that long term estrogen deficiency after ovariectomy, improving lipid profile and blood glucose levels compared to the short time estrogen deficiency during ovariectomized rats.