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Damage intensity of gallic acid on prostatic cancer cells lineDu145 by alkaline electrophoresis
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| Javad Saffari-Chaleshtori , Mostafa Gholami , Mahnaz Keloushadi , Ehsan Heidari , Esfandiar Heidarian |
| Professor Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, I.R. Iran , j_saffari@yahoo.com |
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Abstract: (4007 Views) |
| Background and aims: Prostatic cancer is one of the most widespread cancers in men in all over the world. There are many reagents that cause to prostate cancer, but many factors recognized to prevent or treat this cancer. This capacity is in anti-oxidants and, especially in polyphenol compounds like Gallic acid. This study was aimed to investigate the effect of gallic acid on Du145 cells line in prostatic cancer by alkaline electrophoresis technique.
Methods: In this Laboratory experimental study, MTT assay was used to determine the viability of Du145 cells line in the adjacent of different concentrations of gallic acid and IC50. Three concentrations around the IC50 of Gallic acid were treated on cells for 48 hours. Comet pictures have been analyzed using CASP software after alkaline electrophoresis.
Results: 35 µM of Gallic acid for Du145 cells was determined based on probit model with IC50 with MTT test. Rate of tail to head in alkaline electrophoresis for three concentrations around the IC50 (25, 30 and 35) µM of Gallic acid was 6.7±1.3, 13.8±2.6 and 69.4±1.2 percent, respectively.
Conclusion: Gallic acid is one of the most powerful anti-oxidants that can reduce the growth of Du145 cells in prostatic cancer and inhibits the cell growth with destroy their genome and induce the apoptosis. |
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| Keywords: Prostatic cancer, Du145 cells line, Gallic acid, Comet assay |
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Full-Text [PDF 251 kb]
(650 Downloads)
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Type of Study: Research |
Subject:
genetic
Received: 2014/11/16 | Accepted: 2015/01/20 | Published: 2015/07/24
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