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:: Volume 20, Issue 5 (9-2018) ::
J Shahrekord Univ Med Sci 2018, 20(5): 45-58 Back to browse issues page
Investigation the antibacterial effect of silver nanoparticles made by nanophytosynthesis alone and in combination with chloramphenicol
Ali Shafaghat *
Dept. of Chemistry, Khalkhal Branch, Islamic Azad University, Khalkhal, I.R. Iran , shafaghata@yahoo.com
Abstract:   (4333 Views)
Background and aims: Nanophytosynthesis is a part of nanobiotechnology science and it is the use of plant secondary metabolic compounds through which the synthesis of different nanoparticles is carried out. The resistance of microbes to conventional synthetic antibiotics is inevitable. To reduce this resistance, effective nanoparticles that can have an antibiotic enhancement effect have been used. The aim of this study was to investigate the effect of enhancing the antibiotic properties of chloramphenicol using nanoparticles made by nanophytosynthesis.
Methods: The cubic nanocrystallites of silver were synthesized by nanophytosynthesis method with utilization of the aerial part of Anthemis talyschensis methanolic extract. The structure and optical properties were investigated by FT-IR, UV/Vis, TEM, SEM and XRD. Antibacterial activity of synthesized silver nanocrystallites as separately and in combined with chloramphenicol against four bacterial species were studied according to the disk diffusion method.
Results: The results showed that more than 75% of the synthesized silver nano particles have a cubic structure. The biological study showed that these nanoparticles have a good activity against growth of bacteria and enhances the antibacterial effect of chloramphenicol as antibiotic.
Conclusion: Increasing the inhibition of bacterial growth by these nanocrystals was associated with the silver nano cubic structure. Also, nanophytosynthesis method for different nanoparticle preparation is an economical and green synthesis method, and it is in the adjustment of nature which it provides an optimal condition for production of nanoparticles.
Keywords: Nanophytosynthesis, Antibacterial, Nano silver, Chloramphenicol, Anthemis talyschensis
Full-Text [PDF 1326 kb]   (767 Downloads)    
Type of Study: Research | Subject: medical plants
Received: 2017/10/2 | Accepted: 2018/01/24 | Published: 2018/10/16
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Shafaghat A. Investigation the antibacterial effect of silver nanoparticles made by nanophytosynthesis alone and in combination with chloramphenicol. J Shahrekord Univ Med Sci. 2018; 20 (5) :45-58
URL: http://journal.skums.ac.ir/article-1-3527-en.html

Volume 20, Issue 5 (9-2018) Back to browse issues page
مجله دانشگاه علوم پزشکی شهرکرد Journal of Shahrekord University of Medical Sciences
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