[Home ] [Archive]   [ فارسی ]  
:: Main About Current Issue Archive Search Submit Contact ::
:: Volume 19, Issue 6 (2-2018) ::
J Shahrekord Univ Med Sci 2018, 19(6): 24-37 Back to browse issues page
Effects of silver nanoparticles (Ag-NPs) on testis histology and hormones of pituitary-testicular axis in small laboratory mice (Mus musculus)
Mohammad Saeed Heydarnejad *, Goli Ghasemi , Jahangir Kaboutari
Zoology Dept., University of Shahrekod, Shahrekod, I.R. Iran , m_heydarnejad@yahoo.com
Abstract:   (242 Views)
Background and aims: Regarding Ag-NPs are able to pass through biological memebranes. So, this study aimed to investigate effects of silver nanoparticles (Ag-NPs) on testis histology and hormones of pituitary-testicular axis in small laboratory mice (Mus musculus).
Methods: In this experimental study, a group of 36 BALB/c adult male mice (average weights; 28.5±3 g) were randomly divided into three groups; one control and two treatment groups (n=12 each). The treated groups 1 and 2 received 20 and 50 ppm concentrations of Ag-NPs, respectively. Sampling was done on days 7, 21 and 40 of the experiment. Four groups of mice in each group were anesthetized by anesthetic substance (10 m/L ketamine, 0.5 m/L acepromazine, 2 m/L diazepam and xylazine less than 0.5 m/L) in amounts less than 0.01 m/L by insulin syringe with muscle injections. Blood sampling was obtained directly from the ventricle and serum samples were rotated for 10 min at 3000 rpm. A didgital babalne was used to weigh changes of body weight and biomass of the testes. Histological slides were preapared form the testes and then satined by H and E stining method. The data were analyzed by one-way Anova using SPSS.
Results: The average of mice body weight did not show any significant difference. Howvere, remarkable histological changes were found in the 50-ppm Ag-NPs treated group than to 20 ppm treated group. Serum level of LH increased 8% and 31% in the treated groups one and two, respectively. Howver, serum level of FSH did not show any significant differnec between both tratement groups and the control.
Conclusion: The use of Ag-NPs can lead to hormonal and histological changes in the male testis and consequently affects the male reproductive system and fertility.
Keywords: Testis, Ag-NPs, FSH, LH, Mice.
Full-Text [PDF 1495 kb]   (37 Downloads)    
Type of Study: Research | Subject: biology
Received: 2016/10/4 | Accepted: 2016/12/27 | Published: 2018/02/2
Send email to the article author

Add your comments about this article
Your username or Email:

Write the security code in the box >


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Heydarnejad M S, Ghasemi G, Kaboutari J. Effects of silver nanoparticles (Ag-NPs) on testis histology and hormones of pituitary-testicular axis in small laboratory mice (Mus musculus). J Shahrekord Univ Med Sci. 2018; 19 (6) :24-37
URL: http://journal.skums.ac.ir/article-1-3116-en.html


Volume 19, Issue 6 (2-2018) Back to browse issues page
مجله دانشگاه علوم پزشکی شهرکرد Journal of Shahrekord University of Medical Sciences
Persian site map - English site map - Created in 0.051 seconds with 802 queries by yektaweb 3600