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:: Volume 19, Issue 2 (Jun, Jul 2017) ::
J Shahrekord Univ Med Sci 2017, 19(2): 6-16 Back to browse issues page
Detection of Acinetobacter baumannii by PCR-ELISA method
Suzan Amini Rad , Jafar Amani * , Abbas Ali Imani Fooladi , Pardis Saeedi , Mehrdad Moosazadeh Moghadam
Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, I.R. Iran , jafar.amani@gmail.com
Abstract:   (2324 Views)

Background and aims: AcinetobacterBaumannii causes infections such as respiratory tract, urinary tract and blood and wounds infections; especially in intensive care units around the world and it has ability to acquire drug resistance. In order to recognize and track the treatment of these infections, techniques should continuously be modified. Up to now the investigation has not done on the identification and isolation of the bacteria in clinical samples by PCR-ELISA method in Iran, this study was performed to isolate Acinetobacterbaumannii by PCR-ELISA method.

Methods: In this study, standard strains were evaluated using specific primers and probes by PCR-ELISA method. After amplification and labeling gltA gene, labeled products were coated in micro plates and detected using antibodies against digoxigenin conjugated with peroxidase.

Results: The results of PCR-ELISA method showed that 722 bp fragment from conserved gltA gene. This method is a rapid and sensitive method for the detection of bacteria by using specific primer and probs.

Conclusion: The results indicated that PCR-ELISA possesses a greater sensitivity faster in comparison with conventional PCR. This technique also facilitates the investigation more samples easily and the risk of applying this methods is lower than conventional PCR.

Keywords: Acinetobacter baumannii, gltA gene, PCR-ELISA.
Full-Text [PDF 691 kb]   (1654 Downloads)    
Type of Study: Research | Subject: microbiology
Received: 2016/02/13 | Accepted: 2016/08/6 | Published: 2017/06/3
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Amini Rad S, Amani J, Imani Fooladi A A, Saeedi P, Moosazadeh Moghadam M. Detection of Acinetobacter baumannii by PCR-ELISA method. J Shahrekord Univ Med Sci. 2017; 19 (2) :6-16
URL: http://journal.skums.ac.ir/article-1-2802-en.html

Volume 19, Issue 2 (Jun, Jul 2017) Back to browse issues page
مجله دانشگاه علوم پزشکی شهرکرد Journal of Shahrekord University of Medical Sciences
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